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Molecular Mechanisms Controlling neurogenesis

Universidad Complutense de Madrid

Universidad Complutense of Madrid

Main Areas of Interest

The main research areas of our group are:

Neural Stem Cell (NSC) Behavior and molecular mechanisms controlling neurogenesis, neuronal differentiation, survival, and integration in the postnatal cerebellum
Neural Stem Cell Behavior and molecular mechanisms controlling neurogenesis, neuronal differentiation, survival, and integration within existing networks in the adult subependymal zone
Molecular mechanisms controlling the behavior of Neural Stem Cells and their associated lineage progression and neuronal differentiation in the human embryonic cerebral cortex.
Therapeutic applications of the purinergic signaling in brain repair.

Techniques You Will Learn

Experimental techniques available in our lab:

Cellular cultures. We use different experimental models, primary cultures of granule neurons, and postnatal (P0-P5) NSCs in the cerebellum. Likewise, we perform primary cultures of adult NSCs isolated from the murine subependymal zone. Finally, we also hold expertise in cerebral organoid cultures derived from human induced pluripotent stem cells, both 3D and 2D-based cultures.

Microfluorimetric techniques. a) Determination of free cytosolic calcium concentration in single cell by microfluorimetric techniques allows the quantification the intracellular calcium signals after receptor stimulation.

Electrophysiological recordings. To assay neuronal maturation and integration within existing networks

Time-lapse videomicroscopy. We adapt our cell cultures to live imaging followed by single cell tracking as a powerful tool to monitor any event of the cell biology, proliferation, differentiation, survival, or cell fate decision.

Fixation by transcardial perfusion. Cryopreservation of tissue. Cryostat slicing, Vibratome Slicing.

Antibody-dependent techniques. The immunocytofluorescence, immunohistochemical and western-blot techniques are performed daily in our laboratory.

Epifluorescence and Confocal microscopy.

PCR and Real-time quantitative PCR.

In vivo studies: Stereotactic delivery of gain and loss of function constructs in vivo. In vivo models of cortical lesion.

Transcriptome analysis by massive RNA sequencing. The applicant may also acquire experience in big data processing and interpretation.

Mice handling, ethics, and care.

Data analysis (statistics etc) and manuscript preparation.

Curriculum

6-12 weeks curriculum- Research Plan

Fellows will be supported to settle down into their new environment, with suggestion for accommodations as well as to be registered in our department. Likewise, fellows will be allocated in our laboratory (desk and bench space). Full induction (safety, laboratory practices, personal welfare, etc.) will be provided by the University’s designated staff and by myself. I will arrange an initial meeting with the fellow to discuss the objectives of the training and the expected mechanisms of their fulfilment.

Experimental plans with realistic timelines and the most interesting or suitable research lines will then be decided.

The fellow will be fully trained to run each experiment. They will then be allowed to attempt the experiment themselves, with the trainer present during their first run. They will be able to engage with me (supervisor) and other members of the laboratory to discuss any aspects of the general running of the laboratory or specific protocols that they require clarifications on. They will also be trained in how to analyse and interpret their data. I will arrange a weekly or bi-weekly meeting with the fellow to discuss and reflect on their progress and decide the next set of experiments based on data generated. A fellow must keep adequate and accurate records of their work.

The fellow will be given opportunity to partake in research group meetings and other academic activities within the School and the University, including attending relevant bespoke workshops on research career development (e.g., on manuscript writing, grant writing, networking, etc.). They will also be encouraged to present their work orally at a research group meeting at least once before they complete their visit.

Towards the end of the training, I will have a meeting with the fellow to discuss plans to publish their work (if enough data collected) or disseminate their findings in a future conference. They will also be assisted to identify existing or future opportunities, globally or within their original work environment, that the training has empowered them to be eligible and competitive for and how they can be assisted to access them and integrate the knowledge and skills gained into their further research or professional practice roles. All throughout the training any peculiar challenges or specific requests of a fellow will be fully considered to ensure a responsive, relevant, and adequate training plan is developed for them.

Accommodation

Guest house/accommodation near the lab (Most of them walking distance)

University related accommodation:

https://www.ucm.es/english/housing-office

Affordable Hotels nearby:

Hostal Almansa:

https://www.booking.com/Share-ylc4Yf

Hostal Arrate:

https://www.booking.com/Share-12xNko

Hostal Los angeles:

https://www.booking.com/Share-fynUqvm

Additional accommodation might be explored through Airbnb and similar websites.

Fees and Program Logistics

This program has the following durations available:

Duration	Fee
2 weeks	$875.00
6 weeks	$1,500.00
12 weeks	$2,750.00

This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.

Who Can Apply

All are eligible

Host Details

Host Name: Dr.Felipe Ortega

Affiliation: Universidad Complutense de Madrid

Address: Universidad Complutense de Madrid; Av Puerta de Hierro; s/n, 28040; Madrid; Spain

Website URL: https://www.ucm.es/

Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.

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