Trialect

Active

Biomarkers in Celiac Diseases and Polymers and Drug Delivery Research

Gene Expression-Microarrays Lab, Bambino Gesù Children's Hospital

Bambino Gesù Children's Hospital, IRCCS

Main Areas of Interest

The main research areas of our group are:

Identification of circulating biomarkers of Celiac Disease;
Study of biological processes of wound healing and regeneration processes;
Delivery of drugs by polymers and nanovectors.

Activities are:

Gene expression and post-transcriptional regulation by microRNAs (experimental and computational activities) in pediatric diseases.
Non-coding RNAs (RNA-Seq and Small RNA-Seq) in eukaryotes and prokaryotes.
Circulating microRNAs for diagnostic and therapeutic applications.
Exome sequencing of rare pediatric diseases and bioinformatics analysis.
Nanotechnology and polymer science applied to the in vitro and in vivo delivery of nucleic acids.

Techniques You Will Learn

Experimental techniques you will learn during your program here:

Working with biological samples: blood, plasma/serum, urine and feces;
Molecular biology protocols for gene expression, transfection and regulation;
2D and 3D cell cultures: human cells and/or organoids;
3D bioprinting of scaffolds for cell cultures or polymer-embedded cells;
cell culture and transformation (gene silencing, gene overexpression);
cell co-culture models: intestinal, immune and renal cells.
cell viability/toxicity assays (XTT, sulphorodamine)
cell metabolism assays (enzymatic activity by spectrofluorimetric and chemiluminescence-based assays)
chromatography (thin layer chromatography, ion exchange chromatography, affinity chromatography)
RNA extraction and quality control from different biological specimens;
qualitative and quantitative PCR; PCR-arrays;
Bioinformatics analysis of sequencing data;
ELISA
flow cytometry
fluorescence and confocal microscope
immunohistochemistry

Curriculum

6-12 weeks curriculum

Fellows will be supported to settle down into their new environment (find appropriate accommodation, locate amenities, etc.) and register at the Research Laboratory, during which a badge card will be issued. A desk space will be allocated. Full introduction (safety, laboratory practices, personal welfare, etc.) will be provided by the Laboratory’s designated staff and by myself. I will arrange an initial meeting with the fellow to discuss the objectives of the training and the expected mechanisms of their fulfilment. Experimental plans with realistic timelines will then be decided.

The fellow will be fully trained by myself and my laboratory staff to run each experiment. They will then be allowed to attempt the experiment themselves, with the trainer present during their first run. They will be able to engage with me (supervisor) and other members of the laboratory to discuss any aspects of the general running of the laboratory or specific protocols that they require clarifications on. They will also be trained in how to analyse and interpret their data. I will arrange a weekly or bi-weekly meeting with the fellow to discuss and reflect on their progress and decide the next set of experiments based on data generated. A fellow must keep adequate and accurate records of their work using the laboratory book provided.

The fellow will be given opportunity to partake in research group/clinical meetings and other academic activities within the Hospital, including attending relevant bespoke workshops on research career development (e.g., on manuscript writing, grant writing, networking, etc.). They will also be encouraged to present their work orally at a research group meeting at least once before they complete their visit.

Towards the end of the training I will have a meeting with the fellow to discuss plans to publish their work (if enough data collected) or disseminate their findings in a future conference. They will also be assisted to identify existing or future opportunities, globally or within their original work environment, that the training has empowered them to be eligible and competitive for and how they can be assisted to access them and also integrate the knowledge and skills gained into their further research or professional practice roles.

All throughout the training any peculiar challenges or specific requests of a fellow will be fully considered to ensure a responsive, relevant and adequate training plan is developed for them.

Accommodation

Guest house/accommodation near the lab (2-5 minutes walking)

Hello Roma (Bed&Breakfast)

Website: https://helloromabb.com/it/ (https://www.youtube.com/watch?v=wnBVQ0yYkH8)

B&B Mr Brown - San Paolo

Website: https://beb.it/mrbrownsuites

Many other accomodations are available upon request or following AirBnB website (https://www.airbnb.it) specifying an address nearby the Laboratory

Fees and Program Logistics

This program has the following durations available:

Duration	Fee
2 weeks	$875.00
6 weeks	$1,500.00
12 weeks	$2,750.00

This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.

Who Can Apply

The fellow will be fully trained by myself and my laboratory staff to run each experiment.

Host Details

Host Name: ANDREA MASOTTI, Ph.D

Affiliation: Gene Expression-Microarrays Lab, Bambino Gesù Children's Hospital

Address: Bambino Gesù Children's Hospital, IRCCS; Research Laboratories - V.le di San Paolo 15; 00146 ;Rome - ITALY

Website URL: https://www.ospedalebambinogesu.it/

Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.

Legal & Affiliation Disclaimer

Affiliation Disclaimer: Trialect operates independently and is not affiliated with, endorsed by, or supported by any sponsors or organizations posting on the GrantsBoard platform. As an independent aggregator of publicly available funding opportunities, Trialect provides equal access to information for all users without endorsing any specific funding source, content, organization, or sponsor. Trialect assumes no responsibility for the content posted by sponsors or third parties.

Subscription Disclaimer: Upon logging into Trialect, you may choose to SUBSCRIBE to GrantsBoard for timely notifications of funding opportunities and to access exclusive benefits, such as priority alerts, reminders, personalized recommendations, and additional application support. However, users are advised to contact sponsors directly for any questions and are not required to subscribe to engage with funding opportunities.

Content Ownership and Copyright Disclaimer: Trialect respects the intellectual property rights of all organizations and individuals. All content posted on GrantsBoard is provided solely for informational purposes and remains the property of the original owners. Trialect does not claim ownership of, nor does it have any proprietary interest in, content provided by third-party sponsors. Users are encouraged to verify content and ownership directly with the posting sponsor.

Fair Use Disclaimer: The information and content available on GrantsBoard are compiled from publicly accessible sources in alignment with fair use principles under U.S. copyright law. Trialect serves as an aggregator of this content, offering it to users in good faith and with the understanding that it is available for public dissemination. Any organization or individual who believes their intellectual property rights have been violated is encouraged to contact us for prompt resolution.

Third-Party Posting Responsibility Disclaimer: Trialect is a neutral platform that allows third-party sponsors to post funding opportunities for informational purposes only. Sponsors are solely responsible for ensuring that their postings comply with copyright, trademark, and other intellectual property laws. Trialect assumes no liability for any copyright or intellectual property infringements in third-party content and will take appropriate action to address any substantiated claims.

Accuracy and Verification Disclaimer: Trialect makes no warranties regarding the accuracy, completeness, or reliability of the information provided by sponsors. Users are advised to verify the details of any funding opportunity directly with the sponsor before taking action. Trialect cannot be held liable for any discrepancies, omissions, or inaccuracies in third-party postings.

Notice and Takedown Policy: Trialect is committed to upholding copyright law and protecting the rights of intellectual property owners. If you believe that content on GrantsBoard infringes your copyright or intellectual property rights, please contact us with detailed information about the claim. Upon receipt of a valid notice, Trialect will promptly investigate and, where appropriate, remove or disable access to the infringing content.