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Biochemistry and Metastatic Signaling

University of Patras

Galatea Kallergi is Associate Professor of Biochemistry, Department of Biology,
University of Patras, Greece.Her research focuses on cancer biology, particularly the detection and characterization of circulating tumor cells (CTCs), development of liquid biopsy diagnostic tools, and the study of micrometastatic disease.

Main Areas of Interest

Main Areas of Interest:

Overview of Liquid Biopsy (LB)
Types of analytes: Circulating Tumor Cells (CTCs) and Extracellular
Vesicles (EVs).
Characteristics and properties of CTCs and EVs.
Role of CTCs and EVs in cancer progression, metastasis, and disease
monitoring.
Liquid Biopsy Applications in Cancer Research
Isolation and molecular characterization of CTCs and EVs for disease monitoring.
Integrating LB techniques to influence treatment decisions and improve patient
outcomes.
Progressing precision oncology through utilizing LB in personalized medicine.

Specialties

Liquid biopsy
Circulating Tumor Cells (CTCs)
Extracellular Vesicles (EVs)
Cancer metastasis
Precision Oncology

Specialties

Biochemistry

Physiology

Cancer

Techniques You Will Learn

Techniques you will learn:
1. General knowledge of Liquid Biopsy

Isolation and Enrichment of CTCs and EVs: Techniques for
isolating CTCs and EVs from blood samples, using size-based
filtration, microfluidic platforms and density gradient methods.
Immunofluorescence (IF) Staining: Methods for phenotypic
characterization, using established IF staining panels.

2. Molecular and Functional Analysis

Microscopy and Imaging Techniques: Training in high-resolution
imaging for detailed visualization of CTC morphology and EV
structure.
Molecular Analysis: Techniques such as immunostaining, qPCR and
Immunoblotting for studying molecular profiles in CTCs and EVs.

3. Technology Development

Microfluidic Platforms: Hands-on experience with microfluidic
systems for improved cancer cell culture and characterization.
Multi-Step Assay Development: Techniques for designing sequential
assays to analyze distinct features in CTCs and EVs from the same
sample, enhancing diagnostic insights.

4. Drug Response and Therapeutic Investigation

Functional Assays for Investigating Drug Impact: Techniques for
assessing the effects of anti-cancer drugs on cell viability, apoptosis
and invasion potential in cancer and patient-derived cell lines.
Translation to Cultured CTCs: Developing protocols to adapt drug
response studies for cultured CTCs derived from patient samples.

Curriculum

Curriculum
2-week in-person laboratory study. Details will be provided to the trainees.

Accommodation

Accommodation

The participant will arrange their own accommodations close location to the institute.
You may also consider options on Airbnb.com for short-term stays.

Fees and Program Logistics

This program has the following durations available:

Duration	Fee
2 weeks	$875.00

Who Can Apply

Biomedical sciences and Physicians who have completed their graduation

Host Details

Host Name: Dr.Galatea Kallergi

Affiliation: University of Patras

Address: University of Patras, 26504 Rio, Greece

Website URL: https://www.upatras.gr/en/university/

Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.

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