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Virtual CRISPR Gene Editing Mentorship Program on Hold; pending review
The Virtual CRISPR Gene Editing Mentorship Program is an interactive, one-on-one virtual learning experience designed to provide in-depth knowledge and hands-on training in CRISPR gene editing. Hosted by Dr. Cihan Taştan at the Üsküdar University Transgenic Cell Technologies and Epigenetic Application and Research Center, this program offers participants the opportunity to learn cutting-edge techniques, engage in live demonstrations, and receive personalized mentorship from an expert in the field.
Ideal for researchers, students, and professionals looking to advance their skills in gene editing, this program emphasizes practical applications, problem-solving, and innovative research in a supportive and dynamic virtual environment. Participants will gain a thorough understanding of CRISPR mechanisms, explore real-life applications, and discuss ethical considerations, ensuring a comprehensive learning experience tailored to their individual needs and interests.
Main Areas of Interest
1. Introduction to CRISPR Technology:
- Overview of CRISPR/Cas systems and their discovery.
- Fundamental principles of CRISPR gene editing.
- Comparison with other gene editing technologies.
2. Mechanisms and Techniques:
- Detailed explanation of how CRISPR/Cas9 works at the molecular level.
- Guide RNA design and synthesis.
- Delivery methods for CRISPR components into cells.
3. Applications of CRISPR:
- Use of CRISPR in basic research, agriculture, and medicine.
- Case studies highlighting successful CRISPR applications.
- Potential future applications and innovations.
4. Hands-On Demonstrations:
- Live demonstrations of CRISPR gene editing techniques.
- Step-by-step walkthroughs of experimental procedures.
- Virtual lab simulations for practical experience.
5. Ethical and Regulatory Considerations:
- Discussion of the ethical implications of gene editing.
- Overview of current regulations and guidelines.
- Debate on the societal impact and future of CRISPR technology.
6. Troubleshooting and Problem-Solving:
- Common challenges and solutions in CRISPR experiments.
- Tips for optimizing experimental conditions and outcomes.
- Personalized advice for specific research projects.
7. Innovative Research and Development:
- Exploration of cutting-edge research in CRISPR technology.
- Discussion on the role of CRISPR in novel therapeutic approaches.
- Encouragement of innovative thinking and project development.
8. Personalized Mentorship:
- One-on-one guidance and support from Dr. Cihan Taştan.
- Tailored learning plans to meet individual goals and interests.
- Opportunities for feedback and continuous improvement.
This comprehensive program ensures participants gain both theoretical knowledge and practical skills, preparing them to contribute to the exciting and rapidly evolving field of gene editing.
Techniques You Will Learn
1. Guide RNA (gRNA) Design and Synthesis:
- Principles of designing effective gRNAs.
- Tools and software for gRNA design.
- Synthesis and validation of gRNAs.
2. CRISPR/Cas9 Delivery Methods:
- Techniques for delivering CRISPR components into cells.
- Use of viral vectors, plasmids, and ribonucleoprotein complexes.
- Electroporation, lipofection, and microinjection methods.
3. Gene Editing Workflow:
- Preparation and assembly of CRISPR/Cas9 components.
- Target cell culture and preparation.
- Transfection protocols for various cell types.
4. Screening and Validation:
- Methods for detecting and analyzing CRISPR-induced mutations.
- Polymerase Chain Reaction (PCR) and gel electrophoresis.
- Next-generation sequencing (NGS) for detailed analysis.
5. Off-Target Analysis:
- Techniques to assess and minimize off-target effects.
- Use of computational tools and databases for off-target prediction.
- Experimental validation of off-target activity.
6. Genome Editing Efficiency Enhancement:
- Strategies to improve CRISPR editing efficiency.
- Use of chemical enhancers and optimized delivery conditions.
- Co-delivery of repair templates for homology-directed repair (HDR).
7. Functional Genomic Screens:
- Design and execution of CRISPR screens to identify gene function.
- Use of pooled gRNA libraries.
- Analysis and interpretation of screen results.
8. CRISPR-Based Transcriptional Regulation:
- Techniques for CRISPR activation (CRISPRa) and inhibition (CRISPRi).
- Design of CRISPR systems for gene expression modulation.
- Applications in studying gene regulatory networks.
9. Live-Cell Imaging of CRISPR Activity:
- Techniques for visualizing CRISPR/Cas9 activity in real-time.
- Use of fluorescently labeled Cas9 and gRNA.
- Applications in tracking gene editing events.
10. CRISPR for Epigenetic Modifications:
- Use of CRISPR tools for targeted epigenome editing.
- Applications in studying and manipulating epigenetic marks.
- Techniques for CRISPR-based chromatin modification.
These techniques ensure that participants gain practical skills and hands-on experience in the most relevant and advanced aspects of CRISPR gene editing, preparing them to apply these methods in their own research and professional projects.
Modules
Module 1: Introduction to CRISPR Technology
- Overview of CRISPR/Cas systems and their history.
- Fundamental principles and mechanisms of CRISPR gene editing.
- Comparison with other gene editing technologies.
Module 2: Guide RNA (gRNA) Design and Synthesis
- Principles of effective gRNA design.
- Tools and software for designing gRNAs.
- Synthesis, validation, and optimization of gRNAs.
Module 3: CRISPR/Cas9 Delivery Methods
- Techniques for delivering CRISPR components into cells.
- Viral vectors, plasmids, and ribonucleoprotein (RNP) complexes.
- Electroporation, lipofection, and microinjection methods.
Module 4: Gene Editing Workflow
- Preparation and assembly of CRISPR/Cas9 components.
- Target cell culture and preparation.
- Transfection protocols for various cell types.
Module 5: Screening and Validation of Gene Edits
- Methods for detecting and analyzing CRISPR-induced mutations.
- PCR and gel electrophoresis.
- Next-generation sequencing (NGS) for detailed mutation analysis.
Module 6: Off-Target Analysis and Minimization
- Assessing and minimizing off-target effects.
- Computational tools and databases for off-target prediction.
- Experimental validation techniques for off-target activity.
Module 7: Enhancing Genome Editing Efficiency
- Strategies to improve CRISPR editing efficiency.
- Chemical enhancers and optimized delivery conditions.
- Co-delivery of repair templates for homology-directed repair (HDR).
Module 8: Functional Genomic Screens
- Design and execution of CRISPR screens to identify gene function.
- Pooled gRNA libraries and their applications.
- Analysis and interpretation of screen results.
Module 9: CRISPR-Based Transcriptional Regulation
- Techniques for CRISPR activation (CRISPRa) and inhibition (CRISPRi).
- Design of CRISPR systems for gene expression modulation.
- Applications in studying gene regulatory networks.
Module 10: Live-Cell Imaging of CRISPR Activity
- Techniques for visualizing CRISPR/Cas9 activity in real-time.
- Use of fluorescently labeled Cas9 and gRNA.
- Applications in tracking gene editing events.
Module 11: CRISPR for Epigenetic Modifications
- Use of CRISPR tools for targeted epigenome editing.
- Applications in studying and manipulating epigenetic marks.
- Techniques for CRISPR-based chromatin modification.
Module 12: Ethical and Regulatory Considerations
- Ethical implications of gene editing technologies.
- Current regulations and guidelines governing CRISPR use.
- Societal impacts and future considerations of CRISPR technology.
Module 13: Personalized Mentorship and Project Development
- One-on-one guidance and support from Dr. Cihan Taştan.
- Tailored learning plans based on individual goals and interests.
- Advice on developing and executing CRISPR research projects.
Each module is designed to build upon the previous one, ensuring a comprehensive understanding and practical proficiency in CRISPR gene editing techniques and applications.
Program Fee
| Total Number of Modules | 12 |
| Time duration of Each module | 40 minutes |
| Total Program Cost | $600 |
This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.
Who Can Apply
1. Educational Background:
- A minimum of a bachelor's degree in a relevant field such as biology, biotechnology, genetics, molecular biology, biochemistry, or a related discipline.
- Advanced degrees (Master’s, Ph.D., or equivalent) are preferred but not required.
2. Professional Experience:
- Researchers, scientists, and professionals currently working in the fields of genetic engineering, molecular biology, biotechnology, or related areas.
- Students enrolled in graduate or postgraduate programs related to life sciences and biomedical research.
- Healthcare professionals and clinicians interested in applying gene editing technologies in medical practice.
3. Technical Skills:
- Basic laboratory skills and familiarity with standard molecular biology techniques (e.g., PCR, gel electrophoresis, cell culture).
- Understanding of genetic concepts and terminology.
4. Interest and Motivation:
- Demonstrated interest in gene editing technologies and their applications.
- Motivation to engage in hands-on training and apply CRISPR techniques in research or professional practice.
5. Language Proficiency:
- Proficiency in English, as the program will be conducted in English.
6. Access to Resources:
- Reliable internet connection for participating in live virtual sessions.
- Availability of a computer or device capable of supporting video conferencing and online learning tools.
Applicants who meet these criteria are encouraged to apply for the program, as it is designed to enhance their knowledge and skills in CRISPR gene editing, providing a strong foundation for advanced research and professional development in this cutting-edge field.
Host Details
Host Name: Cihan Tastan
Affiliation: Üsküdar University
Address: Altunizade, Universite Sk. No:14 Üsküdar Istanbul
Website URL: https://uskudar.edu.tr/en
Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.
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Live Interactive One-On-One (Virtual)
Fellowship - Basic/Translational/Clinical Research Program
Turkey
Application Review Deadline:
Dec 1st, 2024
Program Logistics and Administrative Fees
This program has the following durations available:
| Duration | Fee |
|---|---|
| 2 weeks | $625.00 |
| 6 weeks | $1,000.00 |
| 12 weeks | $1,500.00 |
This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.
Who Can Apply
1. Educational Background:
- A minimum of a bachelor's degree in a relevant field such as biology, biotechnology, genetics, molecular biology, biochemistry, or a related discipline.
- Advanced degrees (Master’s, Ph.D., or equivalent) are preferred but not required.
2. Professional Experience:
- Researchers, scientists, and professionals currently working in the fields of genetic engineering, molecular biology, biotechnology, or related areas.
- Students enrolled in graduate or postgraduate programs related to life sciences and biomedical research.
- Healthcare professionals and clinicians interested in applying gene editing technologies in medical practice.
3. Technical Skills:
- Basic laboratory skills and familiarity with standard molecular biology techniques (e.g., PCR, gel electrophoresis, cell culture).
- Understanding of genetic concepts and terminology.
4. Interest and Motivation:
- Demonstrated interest in gene editing technologies and their applications.
- Motivation to engage in hands-on training and apply CRISPR techniques in research or professional practice.
5. Language Proficiency:
- Proficiency in English, as the program will be conducted in English.
6. Access to Resources:
- Reliable internet connection for participating in live virtual sessions.
- Availability of a computer or device capable of supporting video conferencing and online learning tools.
Applicants who meet these criteria are encouraged to apply for the program, as it is designed to enhance their knowledge and skills in CRISPR gene editing, providing a strong foundation for advanced research and professional development in this cutting-edge field.
Host Details
Host Name: Cihan Tastan
Affiliation: Üsküdar University
Address: Altunizade, Universite Sk. No:14 Üsküdar Istanbul
Website URL: https://uskudar.edu.tr/en
Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.
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