Trialect

Active

Signal Transduction in Oncology and Genetic Myopathies

Mario Negri Institute for Pharmacological Research, Milan, Italy

Main Areas of Interest

The main research areas of our group are:

Molecular basis of genetic myopathies;
Molecular basis of Triple Negative Breast cancer;
Studies of Endoplasmic Reticulum Stress Response in disease pathogenesis.

Techniques You Will Learn

Experimental techniques you will learn during your program here:

2D cell culture using: human and murine cells; primary culture of mouse embryonic fibroblasts and myoblasts/myotubes
cell culture and transformation (gene silencing, gene overexpression) by using viral and CRISPR/CAS9 approaches
cell viability/toxicity and migration assays (necrosis, apoptosis, cell cycle analysis, senescence, Boyden Chamber assay)
cell metabolism assays (deoxyglucose uptake)
immunoblotting, co-immunoprecipitation
cell subfractioning
qualitative and quantitative PCR;
ELISA
fluorescence, confocal and super-resolution microscopy
immunohistochemistry on frozen tissue sections
oncological mouse models: mouse models of breast cancer, orthotopic tumor transplantation, mice handling, mice genotyping, tumor monitoring, drug administration
mouse models of genetic myopathies: mice handling, mice genotyping, drug administration, assessment of muscle function, gene delivery by adeno-associated virus injection in muscle.

Curriculum

6 months Curriculum

Fellows will be supported to settle down into their new environment and register at the Mario Negri Institute, during which an ID card will be issued. A desk space will be allocated. Full induction (safety, laboratory practices, personal welfare, etc.) will be provided by the Institute’s designated staff and by myself. I will arrange an initial meeting with the fellow to discuss the objectives of the training and the expected mechanisms of their fulfillment. Experimental plans with realistic timelines will then be decided.

The fellow will be fully trained to run each experiment. They will then be allowed to attempt the experiment themselves, with the trainer present during their first run. They will be able to engage with me (supervisor) and other members of the laboratory to discuss any aspects of the general running of the laboratory or specific protocols that they require clarifications on. They will also be trained in how to analyze and interpret their data. I will arrange a weekly or bi-weekly meeting with the fellow to discuss and reflect on their progress and decide the next set of experiments based on data generated. A fellow must keep adequate and accurate records of their work using the laboratory book provided.

The fellow will be given opportunity to partake in research group meetings and other academic activities. They will also be encouraged to present their work orally at a research group meeting at least once before they complete their visit.

Towards the end of the training I will have a meeting with the fellow to discuss plans to publish their work (if enough data collected) or disseminate their findings in a future conference. They will also be assisted to identify existing or future opportunities.

All throughout the training any peculiar challenges or specific requests of a fellow will be fully considered to ensure a responsive, relevant and adequate training plan is developed for them.

Accommodation

Try Airbnb

Guest house/accommodation near the lab (2 minutes walking)

Mario Negri guesthouse

website (Italian; English): http://www.marionegri.it

Fees and Program Logistics

This program has the following durations available:

Duration	Fee
2 weeks	$875.00
6 weeks	$1,500.00
12 weeks	$2,750.00

This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.

Who Can Apply

Scientists from around the world can apply

Host Details

Host Name: Prof. Ester Zito

Affiliation: Mario Negri Institute for Pharmacological Research, Milan, Italy

Address: La Masa, 19 - 20156 Milano, Italy

Website URL: https://www.marionegri.it/laboratories/laboratory-of-signal-transduction

Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.

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Onsite/On-Campus Program

Fellowship - Basic/Translational/Clinical Research Program

Italy

Application Review Deadline:

Dec 1st, 2024

Apply Now

Program Logistics and Administrative Fees

This program has the following durations available:

Duration	Fee
2 weeks	$875.00
6 weeks	$1,500.00
12 weeks	$2,750.00

Questions and Answers

Commonly asked questions about this program from the host and other attendees.

Which visa should i apply for?

You have to contact the Italy Embassy in your country or the host institute. In the past, Student visa was considered by some

is this programme also for triple negative breaat cancers

All of the details are already listed in the posting.

How can I apply for this trining?

For more information about the program, please visit our website and apply at https://trialect.com/program-list and attach your updated CV and letter of intent.