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Bone Diseases Clinical, Basic and Translational Research

IRCCS Ospedale Galeazzi - Sant'Ambrogio

Galeazzi Orthopaedic Research Institute, Milan, Italy

Main Areas of Interest

IRCCS Istituto Ortopedico Galeazzi (short name: IOG). is the #1 orthopaedic institute in Italy in terms of surgery volume and the #2 in terms of scientific production. It is recognized by the Italian Ministry of Health as a research institute (IRCCS) from 2006. IOG belongs to the San Donato Hospital & University (GSD), the biggest Italian hospital group, including 18 hospitals 3 of which recognized as IRCCS.

The IOG facilities include: up-to-date equipment for biochemical and biomolecular analyses and for handling, processing, and storing biological samples. The lab is equipped with: biological hoods and air incubators dedicated to eukaryotic (primary, lines) and prokaryotic cells, equipment for molecular biology analyses (thermocyclers, real- time PCR, electrophoretic apparatuses, blotting apparatuses, imaging system), a histology facility, confocal laser scanning microscope, fluorescence microscopes with live acquisition systems and dedicated softwares, automated ELISA and multiplex processors. The GSD group makes fully available to the employees the most up-to-date facilities for histology, microscopy, cytofluorometry, mass spectroscopy, and next generation sequencing.

The activity of the lab is strictly linked and embedded with the clinical activity of the institution with several ongoing clinical and translational research lines. The research focus of the lab is the study of the endocrine function of bone tissue. Specifically, our studies are addressed at investigating how different stimuli to the skeleton modify the endocrine signaling from bone to the organs/tissues involved in energy metabolism (β-cell, adipose tissue, skeletal muscle) and calcium homeostasis (parathyroid glands). This kind of study is performed both in vitro, through the use of cell models, and in vivo in human subjects. The in vitro studies are drawn in order to study the effects of specific molecules on the function/activity of target cells. In particular, we are currently studying the effects of bone-derived molecules on the glucose sensitivity of β-cell and on the calcium sensing of parathyroid glands cells. On the other hand, we are studying the effects of myokines on the differentiation efficiency and the functionality of osteoclasts derived from peripheral blood mononuclear cells.

In vivo we are studying the changes of bone derived markers and hormones, in relation to the changes in energy metabolism-regulating hormones, in subjects submitted to high energy-demanding activities and different degree of load onto the skeleton. A specific aspect is the evaluation of the changes in total circulating and fracture risk-associated miRNAs (both free and microencapsulated) following specific stimuli (e.g., specific protocol of physical activity). Another leading research area is on the study of the endocrine effects of whole-body cryotherapy, a brief exposure to extreme cold air with anti-inflammatory and analgesic effects, widely used in sports medicine and to treat painful symptoms of inflammatory conditions.

Techniques You Will Learn

The principal techniques used in the lab are the following: establishment and propagation of cell line culture (tumor, hybridomas), establishment and propagation of primary cell cultures (osteoblasts, osteoclasts, adipocytes, β-cell, parathyroid gland adenocarcinoma, mesenchymal stem cells, articular chondrocytes, intervertebral disc chondrocytes), bi-dimensional and tri-dimensional cell cultures, agarose gel electrophoresis and gene expression analysis (PCR, real time PCR), restriction fragment length polymorphisms (RFLP) analysis, protein expression analysis (polyacrylamide gel electrophoresis, western blotting), molecular cloning, protein expression in bacteria, overexpression and silencing of target genes or RNA, free and conjugated nucleic acids (DNA, RNA, miRNAs, lncRNA) extraction and analysis from blood and surgical material, protein extraction and analysis from surgical material, histology, immunohistochemistry (bright field and fluorescent), enzymatic assays, enzyme-linked immunosorbent assays (ELISA), multiplex assays (Luminex), biostatistical analysis.

Based on the fellow inclination, the lab can offer different kind of research activities ranging from pure basic to translational and clinical research.

The laboratory has several national and international cooperation and among them: CHU-Sainte Justine Hospital (Montreal, QC, Canada), China Institute of Sports Science (Beijing, PRC), Gdansk University of Physical Education and Sport (Gdansk, Polska), Universidad Catolica de Valencia (Valencia, Espana), Research Institute Hospital 12 de Octubre “i+12” (Madrid, Espana), San Raffaele Hospital (Milano, Italy), University of Milano (Milano, Italy), University of Milano-Bicocca (Milano, Italy).

Curriculum

Can be Customized

Accommodation

Try Airbnb

Accommodation and living expenses will come to around $150 per week - you have to take care of them

Fees and Program Logistics

This program has the following durations available:

Duration	Fee
2 weeks	$875.00
6 weeks	$1,500.00
12 weeks	$2,750.00

This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.

Who Can Apply

All scientists, Phd students, Medical students interested in exposure to research in bone diseases can apply

Host Details

Host Name: Prof. Giovanni Lombardi

Affiliation: IRCCS Ospedale Galeazzi - Sant'Ambrogio

Address: Via Caterina Belgioioso 173, 20157, Milano, Italia

Website URL: https://galeazzi.grupposandonato.it

Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.

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