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Advanced Optical Microscopy
(5/5) - 1 reviews
Maastricht University
Main Areas of Interest
Super-Resolution Imaging of Mitochondria
- Explore advanced imaging techniques to study mitochondrial structure and function at the nanoscale level.
- Investigate mitochondrial dynamics and their role in cellular metabolism and diseases.
Nuclear Lamina in Laminopathies
- Utilize super-resolution microscopy to examine the nuclear lamina in cells affected by laminopathies.
- Understand the structural changes and their implications in genetic disorders.
Uptake of Extracellular Vesicles by Endothelial Cells
- Study the mechanisms of extracellular vesicle uptake and their impact on endothelial cell function.
- Explore the role of extracellular vesicles in cell communication and disease.
Multiphoton Imaging of Cleared Tissue
- Apply multiphoton imaging techniques to visualize cleared tissues such as human placental or epilepsy tissues.
- Investigate the structural and functional properties of these tissues in health and disease.
About the Host Mentor
Marc A.M.J. van Zandvoort is a full professor at Maastricht University in the Department of Molecular Cell Biology within the Faculty of Health, Medicine and Life Sciences. He also holds a personal chair in Genetics and Cell Biology and is affiliated with the GROW School for Oncology and Reproduction.
Prof. van Zandvoort's research focuses on advanced optical microscopy techniques such as multi-photon microscopy, high-resolution 3D-STED microscopy, and spectral microscopy. These techniques are applied to various biomedical fields, particularly in cardiovascular and oncological research. For instance, his team developed a method to measure the functionality of carotid plaque microvessels in mice using in vivo multi-photon microscopy. Additionally, he has conducted numerous studies involving bimodal imaging, such as combining optical techniques with MRI and ultrasound.
He is actively involved in several professional organizations, serving as a board member of the Dutch Society of Microscopy, the NL-BioImaging-AM initiative, and the conference series Focus on Microscopy. He also leads the EuroBioImaging Node Maastricht.
Prof. van Zandvoort is an experienced educator, mentoring and tutoring students from various disciplines, including medicine, biomedical engineering, and science. His research group includes technicians, PhD students, and postdoctoral researchers based in Maastricht and Aachen.
Specialties
Techniques You Will Learn
Facilities:
- The equipment is installed in the CORE lab of Maastricht University and is part of the EuroBioImaging site “Maastricht Excellence in Imaging”.Methods Used:
Nuance Spectral Microscopy
- Enables differentiation of multiple fluorescent signals in complex biological samples.
- Ideal for multiplex imaging in a single experiment.
Stimulated Emission Depletion (STED) Microscopy
- Provides super-resolution imaging by overcoming the diffraction limit of light.
- Allows visualization of structures at the nanometer scale.
Confocal Microscopy
- High-resolution optical imaging technique that increases optical resolution and contrast.
- Constructs 3D images from the optical sectioning of specimens.
Two-Photon Laser Scanning Microscopy
- Enables deep tissue imaging with minimal photodamage.
- Suitable for live tissue and intravital imaging studies.
Advanced Microscopy Program
Basic Microscopy
- Introduction to the principles and techniques of microscopy.
Fluorescence Microscopy
- Utilizes fluorescence to visualize and study biological specimens.
Confocal Laser Scanning Microscopy
- Focuses on confocal techniques for detailed 3D imaging.
Light Sheet Microscopy
- Allows fast and gentle imaging of live organisms and large samples.
High-Throughput Microscopy
- Automation and large-scale imaging for screening applications.
Super-Resolution Microscopy
- Techniques that surpass the diffraction limit of light, with an emphasis on STED.
Multiphoton Laser Scanning Microscopy
- Advanced two-photon techniques for deep tissue imaging.
Vital Imaging of Cells and Tissues
- Real-time imaging of live cells and tissues.
Fluorescence Resonance Energy Transfer (FRET)
- Measures distances between molecules and detects molecular interactions.
Fluorescence Lifetime Imaging (FLIM)
- Provides contrast based on the fluorescence lifetime rather than intensity.
Sample Preparation and Clearing
- Techniques to prepare and clear samples for optimal imaging results.
3D Image Restoration and Image Processing
- Methods to restore and process 3D images for enhanced analysis.
Curriculum
1 week/ 6 weeks/ 12 weeks - can be customized- a representative sample is given below and may not be applicable to you
12-Week Curriculum: General Outline
Week 1: Orientation and Literature Review
- Introduction to optical microscopy techniques and applications.
- Review of relevant literature on imaging techniques and specific research topics.
Week 2: Imaging Techniques - Programs
- Intensive course on advanced imaging techniques, including hands-on sessions.
Weeks 3-4: Microscope Handling and Initial Experiments
- Training on handling various microscopes and performing initial experiments.
- Introduction to ethical considerations in imaging research.
Weeks 5-10: Research Projects
- Embark on specific research projects such as super-resolution imaging of mitochondria, nuclear lamina studies, or multiphoton imaging of cleared tissues.
- Conduct experiments, collect data, and analyze results.
- Opportunity to bring and work on own samples.
Week 11: Data Analysis and Report Writing
- Compile and analyze data from research projects.
- Write a scientific report summarizing findings and potential implications.
Week 12: Presentation and Future Directions
- Present research findings to peers and mentors.
- Discuss potential for publication and future research directions.
Note: Candidates are encouraged to bring their own samples for personalized research projects. The fellowship provides a comprehensive learning experience, combining theoretical knowledge with practical skills in advanced optical microscopy.
Accommodation
Try Airbnb
Fees and Program Logistics
This program has the following durations available:
| Duration | Fee |
|---|---|
| 2 weeks | $875.00 |
| 6 weeks | $1,500.00 |
| 12 weeks | $2,750.00 |
This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.
Who Can Apply
All interested biomedical scientists from around the world are eligible
Host Details
Host Name: Marc A.M.J van Zandvoort
Affiliation: Maastricht University
Address: Minderbroedersberg 4-6, 6211 LK Maastricht, Netherlands
Website URL: https://gcb.mumc.nl/phdpostdoc-course-advanced-optical-microscopy-2019
Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.
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Onsite/On-Campus Program
Fellowship - Basic/Translational/Clinical Research Program
Netherlands
Application Review Deadline:
Dec 1st, 2024
Reviews
Although I could follow the students' projects and learn in practice about the techniques, I think it would be best if there were exclusive prepared samples for the traineeship. I stayed for 6 weeks at University of Maastricht for the Advanced Optical Microscopy in Biomedical Research. The professors/mentor hosts that give the traineeship have a lot of experience and knowledge about microscopy, they were friendly, helpful and open to feedback. I was able to follow the students' projects, learn the routine of the research group and experience real life problems and solutions that one could encounter in this field of research. I enjoyed my time at the University of Maastricht, all the staff were super helpful, and I was able to meet very talented researchers and students. I already had some experience with microscopy, but after this traineeship I’ll be much more confident to decide which technique to use in my nexts projects, and I think that is the most valuable lesson that I learned from this course. Overall I think it was a very positive experience that met my expectations. I was very well received by the mentor host research group. I felt part of their team for these 6 weeks. I also had the opportunity to use high-end equipment that I don’t have access to in my local region. The Netherlands and especially the city of Maastricht were very pleasant and definitely will be missed. Although I could follow the students' projects and learn in practice about the techniques, I think it would be best if there were exclusive prepared samples for the traineeship.
Program Logistics and Administrative Fees
This program has the following durations available:
| Duration | Fee |
|---|---|
| 2 weeks | $875.00 |
| 6 weeks | $1,500.00 |
| 12 weeks | $2,750.00 |
This program allows Merit Applications. This program allows merit-based applications for virtual and onsite clinical and research programs. If you are successfully awarded under this category, Trialect or the host mentor will cover the tuition fee only. All applications will be evaluated based on merit. Due to the high level of competition, the chances of being selected under the merit category are quite limited.
Who Can Apply
All interested biomedical scientists from around the world are eligible
Host Details
Host Name: Marc A.M.J van Zandvoort
Affiliation: Maastricht University
Address: Minderbroedersberg 4-6, 6211 LK Maastricht, Netherlands
Website URL: https://gcb.mumc.nl/phdpostdoc-course-advanced-optical-microscopy-2019
Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.
Questions and Answers
Commonly asked questions about this program from the host and other attendees.
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